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KMID : 0380019940090030332
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Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 3 p.332 ~ p.337
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Reversed Micellar Protein Extraction in a Hollow Fiber Membrane Extractor
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Abstract
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Solubilization phenomena of a protein in a reversed micellar solution were investigated and a hollow fiber membrane extractor was tested for reversed micellar protein extraction equipment. Alkaline protease was used as a model protein compound and the reversed micellar solution was consisted of AOT and isooctane. It was found that protein solubilization was strongly influenced by ionic strength and pH. The distribution coefficient of the protease between the aqueous solution and the AOT/isooctane solution was also observed to be as high as 4.0 within the scope of this experiment. A hollow fiber membrane extractor was constructed and tested for the protein extraction. The overall mass transfer cocffi cient at a typical experimental condition of this study was observed to be 6.7 .~, 10-´cm/ s. It was alsu found that the mass transfer resistance on reversed micellar solution was the dominant resistance for the protein transfer.
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